shrna control (Addgene inc)
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shrna control
Shrna Control, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1557 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plko%2E1+control+shrna/pLKO%2E1+puro+(Plasmid+%238453)/pmc12992470-33-33-35
Average 96 stars, based on 1557 article reviews
Shrna Control, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1557 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plko%2E1+control+shrna/pLKO%2E1+puro+(Plasmid+%238453)/pmc12992470-33-33-35
Average 96 stars, based on 1557 article reviews
shrna control - by Bioz Stars,
2026-09
96/100 stars
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1) Product Images from "TDP-43 impairs glycolysis by sequestering hexokinase 1 in amyotrophic lateral sclerosis"
Article Title: TDP-43 impairs glycolysis by sequestering hexokinase 1 in amyotrophic lateral sclerosis
Journal: Acta Neuropathologica
doi: 10.1007/s00401-026-02996-6
Figure Legend Snippet: Pathological TDP-43 elicits glycolytic impairment in cells. a , b Agilent seahorse glycolytic stress test was performed on n = 3 independent biological repeats. Glycolysis and Glycolytic Capacity were measured according to manufacturer’s protocol. ECAR values were normalized to total protein then plotted against time. Individual glycolysis and glycolytic capacity values were normalized to total protein level then to individual control from each biological repeat. a Stable HEK293 cells were overexpressed with either YFP (control), TDP-43 WT or TDP-43 ΔNLS . Data were analyzed by one-way ANOVA followed by post hoc Tukey’s test. b TDP-43 G298S patient iPSC and control iPSC were differentiated into motor neurons with our established protocol. On day 26 following differentiation, cells were treated with MG-132 (1 μM) for 24 h. Data were analyzed by Student’s t-test. c YFP (control), TDP-43 WT or TDP-43 ΔNLS stable cells were transiently transfected with various glycolysis shRNAs. Cell viability was measured via MTT assay. Decreased cell viability in all TDP-43 WT and TDP-43 ΔNLS with various glycolysis shRNA (n = 3). Data were analyzed by one-way ANOVA followed by post hoc Tukey’s test. All data are mean ± SE
Techniques Used: Control, Transfection, MTT Assay, shRNA
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Control:Article Title: Epigenetic Buffering of Proteotoxic Stress by EHMT2 Enables Meningioma Growth Article Snippet: 219 Ac ce pte d M an us cri pt D ow nloaded from https://academ ic.oup.com /noa/advance-article/doi/10.1093/noajnl/vdag037/8483041 by M aria M estriner user on 07 M arch 2026 Manuscript number NOA-D-25-00449R2 10 Immunostained bands were detected using chemiluminescence (Pierce ECL Western 220 Blotting Substrate, 32209, ThermoFisher Scientific; SuperSignal West Pico PLUS 221 Chemiluminescent Substrate, 34577, ThermoFisher Scientific), and images were 222 obtained using either a KODAK Image Station 4000 System or a Li-Cor Odyssey M 223 Imaging System. .. 224 225 Lentivirus production and generation of ATF4 and CHOP knockdown and SirT1 226 overexpression meningioma cell lines 227 Lentiviral small hairpin RNA (shRNA) vectors for ATF4 (TRCN0000013573; 228 TRCN0000013575) and CHOP (TRCN0000007263; TRCN0000364393) as well as 229 MISSION® ORF SirT1 overexpression (TRCN0000487877) and MISSION® TRC3 ORF 230 GFP control (ORFGFP) were purchased from Sigma-Adrich. Article Title: TDP-43 impairs glycolysis by sequestering hexokinase 1 in amyotrophic lateral sclerosis. Article Snippet: The secondary HRP-conjugated antibodies Goat anti-mouse HRP (#31430; WB 1:5000) and goat antirabbit HRP (#31460; WB 1:5000) were purchased from Thermo Fisher Scientific. .. The following constructs pcDNA3.2-YFP (addgene #84910) , pcDNA3.2 -TDP-43 W T-YFP (human) (addgene #84911), pcDNA3.2-TDP-43ΔNLS-YFP (human) (addgene #84912), pLD-puro-Cc-TARDBPA315T_VA Plasmid (human TDP-43 A315T) (addgene #141329), pLD-puro-Cc-TARDBP-WT_VA Plasmid (human TDP-43 WT) (addgene #141327), and Article Title: TDP-43 impairs glycolysis by sequestering hexokinase 1 in amyotrophic lateral sclerosis Article Snippet: The secondary HRP-conjugated antibodies Goat anti-mouse HRP (#31430; WB 1:5000) and goat anti-rabbit HRP (#31460; WB 1:5000) were purchased from Thermo Fisher Scientific. .. The following constructs pcDNA3.2-YFP (addgene #84910), pcDNA3.2-TDP-43 WT -YFP (human) (addgene #84911), pcDNA3.2-TDP-43 ΔNLS -YFP (human) (addgene #84912), pLD-puro-Cc-TARDBP-A315T_VA Plasmid (human TDP-43 A315T) (addgene #141329), pLD-puro-Cc-TARDBP-WT_VA Plasmid (human TDP-43 WT) (addgene #141327), and Article Title: Modulation of the Receptor Tyrosine Kinase TIE2/ Tek Pathway by NRF2 Activation in Neurovascular Endothelial Cells. Article Snippet: Lentiviruses were produced in HEK293T cells co-transfected with 6 μg of pSPAX2 packaging plasmid (12260, Addgene, Watertown, MA, USA), 6 μg of VSV–G envelope protein plasmid pMD2G (12259, Addgene), and 10 μg of the corresponding lentiviral vector, an empty vector (shCtrl) or with the corresponding shRNA plasmids and packaging vectors, or the pLENTI-PURO empty vector control. .. Construct:Article Title: TDP-43 impairs glycolysis by sequestering hexokinase 1 in amyotrophic lateral sclerosis. Article Snippet: The secondary HRP-conjugated antibodies Goat anti-mouse HRP (#31430; WB 1:5000) and goat antirabbit HRP (#31460; WB 1:5000) were purchased from Thermo Fisher Scientific. .. The following constructs pcDNA3.2-YFP (addgene #84910) , pcDNA3.2 -TDP-43 W T-YFP (human) (addgene #84911), pcDNA3.2-TDP-43ΔNLS-YFP (human) (addgene #84912), pLD-puro-Cc-TARDBPA315T_VA Plasmid (human TDP-43 A315T) (addgene #141329), pLD-puro-Cc-TARDBP-WT_VA Plasmid (human TDP-43 WT) (addgene #141327), and Article Title: TDP-43 impairs glycolysis by sequestering hexokinase 1 in amyotrophic lateral sclerosis Article Snippet: The secondary HRP-conjugated antibodies Goat anti-mouse HRP (#31430; WB 1:5000) and goat anti-rabbit HRP (#31460; WB 1:5000) were purchased from Thermo Fisher Scientific. .. The following constructs pcDNA3.2-YFP (addgene #84910), pcDNA3.2-TDP-43 WT -YFP (human) (addgene #84911), pcDNA3.2-TDP-43 ΔNLS -YFP (human) (addgene #84912), pLD-puro-Cc-TARDBP-A315T_VA Plasmid (human TDP-43 A315T) (addgene #141329), pLD-puro-Cc-TARDBP-WT_VA Plasmid (human TDP-43 WT) (addgene #141327), and |